Please use this identifier to cite or link to this item:
https://rsuir-library.rsu.ac.th/handle/123456789/3204| Title: | A retrospective study on the molecular diagnosis of cutaneous non-tuberculous mycobacterial infection compared to conventional culture method at institute of dermatology, Bangkok |
| Authors: | May Soe Htet |
| metadata.dc.contributor.advisor: | Praneet Sujjachareonpong |
| Keywords: | Mycobacterium Infections;Dermatology;Molecular diagnostic techniques |
| Issue Date: | 2024 |
| Publisher: | Rangsit University. Library |
| metadata.dc.description.other-abstract: | With increasing prevalence of cutaneous non-tuberculous mycobacterial (NTM) infection in Thailand, molecular diagnostic methods such as PCR and gene sequencing have played an important role in diagnosis and management of the disease. Although culture is still the gold standard for diagnosis, molecular diagnosis provides faster results enabling prospect of better clinical outcome. We aimed to compare the efficacy of Polymerase Chain Reaction (PCR) and sequencing technique using direct tissue sample to conventional culture method, and also to study the clinical characteristics of cutaneous NTM infections at the Institute of Dermatology. The medical records of all cases with confirmed diagnosis of cutaneous NTM infection during 2016 - 2020, with the results of both culture and PCR/gene sequencing of fresh tissue, formalin-fixed, paraffin-embedded tissue (FFPE) sample were collected. Of all medical records, only 52 cases met our criteria, where only 9 cases had both positive culture and PCR from fresh tissue. Notably, 7 cases had negative culture but positive PCR from fresh tissue while in 36 cases, culture was positive with negative PCR from fresh tissue (p<0.05). Neither the duration of lesions (≤4 weeks or >4 weeks) nor the morphology had any correlation with the molecular result. M. abscessus was the most common pathogen followed by M. marinum. Of note, 32 cases (64%) showed non-specific histological features. Most patients had combination therapy with 2 or 3 antibiotics. Combination of clarithromycin with either ciprofloxacin or co-trimoxazole was commonly used. In conclusion, conventional culture is the gold standard for diagnosis. Negative PCR results in most cases may result from inadequate tissue sampling showing less sensitivity. In addition, our study was limited by the small number of cases during the period of research |
| Description: | Thesis (M.Sc. (Dermatology and Dermatosurgery)) -- Rangsit University, 2024 |
| metadata.dc.description.degree-name: | Master of Science |
| metadata.dc.description.degree-level: | Master's Degree |
| metadata.dc.contributor.degree-discipline: | Dermatology and Dermatosurgery |
| URI: | https://rsuir-library.rsu.ac.th/handle/123456789/3204 |
| metadata.dc.type: | Thesis |
| Appears in Collections: | Med-DD-M-Thesis |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| MAY SOE HTET.pdf | 390.25 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.